Asian Journal of Bio Science

B. T. Kavitha ¹, Y. L. Ramachandra ¹

Affiliations
1 Department of Biotechnology and Bioinformatics, Kuvempu University, Shimoga (Karnataka), IN

Abstract

For centuries, indigenous medicinal plants have been used against bacterial-induced pathogenesis. Hemidesmus indicus is a pharmacologically important plant. The in vitro experiment by the agar well diffusion assay showed the presence of bioactive components in H. indicus leaf extract through zone of inhibition. In different ratios of petroleum ether and ethyl acetate test have shown significant zone of inhibition against gram positive and gram negative bacteria: Bacillus subtilis (54 mm), Escherichia coli (70 mm) and Pseudomonas aeruginosa (50 mm).

Keywords

Antibacterial Activity, H. indicus, Medicinal Plants, Bioactive Components.

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Y. L. Ramachandra ¹, S. Padmalatha Rai ², H. V. Sudeep ¹, P. S. Sujan Ganapathy ¹, B. T. Kavitha ¹, N. B. Krishnamurthy ³

Affiliations
1 Department of Biotechnology and Bioinformatics, School of Biological Sciences, Kuvempu University, Jnana Sahyadri, Shimoga (Karnataka), IN
2 Department of Biotechnology, Manipal Life Science Centre, Manipal University, Manipal (Karnataka), IN
3 Department of Biotechnology, Shridevi Institute of Engineering and Technology, Tumkur (Karnataka), IN

Abstract

Leaf moisture content and moisture retention rate are important qualitative parameters from the point of young age silkworm rearing. In the present investigation relative moisture loss from tender leaves of five selected mulberry varieties viz. S₃₆, S₅₄, DD, V₁ and M₅ from Shimoga district were evaluated. Differences among the five varieties with respect to the leaf moisture retention capacity/moisture loss are included in this study. Based on the values recorded, variety V1 showed higher retention rate at different hours of preservation compared to rest of the varieties. The lowest rate was recorded from M₅ variety.

Keywords

Leaf Moisture Loss, Retention Rate, Mulberry Varieties.

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C. C. Gavimath ¹, Y. L. Ramachandra ¹, S. Padmalatha Rai ², H. V. Sudeep ¹, P. S. Sujan Ganapathy ¹, B. T. Kavitha ¹

Affiliations
1 Department of Biotechnology and Bioinformatics, School of Biological Sciences, Kuvempu University, Jnana Sahyadri, Shimoga (Karnataka), IN
2 Department of Biotechnology, Manipal Life Science Center, Manipal University, Manipal (Karnataka), IN

Abstract

Many herbal remedies individually or in combination have been recommended in various medical treatises for the cure of different diseases. The therapeutic value of Aegle marmelos Correa (Rutaceae) commonly known as ‘Bael’ has been recognized in different system of traditional medication for the treatment of different diseases and ailments of human beings. The phytochemical and antibacterial studies of the leaf extracts of A. marmelos have been investigated. The antibacterial activity was investigated against multi resistant strains of Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Salmonella typhi, Proteus vulgaris, Pseudomonas aeruginosa and Klebsiella pneumoniae by disc diffusion method. The results showed that the antibacterial efficacy of petroleum ether extract of leaf was significant and nearer to the standard antibiotic Streptomycin tested. Further the activity was more pronounced in gram-negative strains and moderate in case of gram-positive strains studied. The study results suggest that this plant is promising in the development of phytomedicine for bacterial diseases.

Keywords

Antibacterial Activity, Bioactive Components, Multi Resistant Strains, Aegle marmelos, Disk Diffusion Method.

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